Polymerase Chain Reaction Cabinets
Germfree manufactures PCR Workstations for processes involving Polymerase Chain Reaction amplification and manipulation of DNA and RNA. We offer a range of PCR cabinets, including units that protect personnel and the work environment while protecting samples from contamination.
Amplification processes are very sensitive and proper sterility levels are required to achieve accurate PCR results. Process contamination and cross-contamination are a serious concern. Therefore, it is imperative that PCR work is conducted within an environment where air quality is controlled. Additionally, certain PCR applications require personnel protection from potential particulate and aerosol hazards.
Germfree manufactures two types of PCR cabinets. Our standard L1 cabinet provides laminar flow supply air to protect the samples in the work area. Our advanced L2 unit provides Vertical Laminar Flow with HEPA Exhaust to protect product, personnel and environment.
Germfree’s PCR Workstations are designed for varied applications including: genetic testing, preimplantation genetic diagnosis, tissue typing, PCR-based oncogene testing, characterization of infectious disease organisms, detection of antibiotic resistance for development of new therapies, detection of disease organism sub-types in animal populations for surveillance of potential epidemics, general forensics applications, genetic fingerprinting protocols, molecular genetics, generation of hybridization probes, DNA cloning, quantitative PCR as well as niche applications such as the phylogenic analysis of DNA in archeology.
PCR-L1: Laminar Flow PCR Cabinet
The PCR-L1 is our standard PCR cabinet that provides HEPA-filtered air to maintain sterility for your samples. The work area is continuously bathed with positive pressure High Efficiency Particulate Air (HEPA) filtered air to protect the product from contamination. Our PCR-L1 meets or exceeds ISO 14644-1, Federal Standard 209E for ISO 5/Class 100 air and IES Recommended Practice for Laminar Flow Clean Air Devices. The laminar flow principle consists of moving individual streams of unidirectional, ultra-clean air along parallel lines with minimal turbulence. The HEPA filter is positioned in the top of the work area and is protected by a removable perforated metal diffuser. Additionally, Germfree provides a timer-controlled UV lamp installed in the interior of the cabinet.
PCR-L2: Class II, Type A PCR Biosafety Cabinet
Advanced PCR Workstation Protects Personnel, Product and Environment
Germfree’s PCR-L2 is a Class II, Type A Vertical Laminar Flow Biological Safety Cabinet. This PCR workstation provides protection for the user, product and the environment from particulate and aerosol hazards. The work area is continuously bathed with positive pressure ISO 5/Class 100 HEPA filtered-air to protect the PCR samples from contamination while an inward airflow protects the operator. Approximately 70% of the air from each cycle is recirculated through the supply HEPA filter while the remaining air is discharged from the hood through the exhaust HEPA filter. Additionally, Germfree provides a timer-controlled UV lamp installed in the interior of the cabinet.
The PCR-L2 passes the Biological Tracer Containment Tests of the National Institutes of Health (NIH), the National Cancer Institute (NCI). All units are NRTL listed with MET Labs to comply with UL1262.
Portable and Durable Units
Germfree is a leading manufacturer of mobile bio-containment laboratories. We have installed our PCR cabinets into various truck and trailer-based platforms. Our PCR cabinets are field-tested making them suitable for the most demanding environments. Germfree is the only company with experience in ruggedized cabinetry to insure that your PCR workstations can be deployed wherever they are needed. Additionally, Germfree products are designed for ease of performance testing and certification of the equipment when required.